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URL: http://www.addgene.org/32242
Proper Citation: RRID:Addgene_32242
Insert Name: A. thaliana tRNA ligase
Organism: Arabidopsis thaliana
Bacterial Resistance: Kanamycin
Defining Citation: PMID:20075163
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5255; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: We have found that this protein expresses best in the BL21 (DE3) RIPL cells from Stratagene. Standard BL21 (DE3) strains that lack tRNAs for rare codons do not work well (or at all).
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Source: Addgene