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URL: http://www.addgene.org/32101
Proper Citation: RRID:Addgene_32101
Insert Name: Metallothionein
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17692533
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6700; Vector Backbone:pMAL-c2x; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The metallothionein (MT) gene was amplified with the forward primer (5'-CTCGGGATCGAGGGAAGGATTTCAAGA TATACCATGGACCCC-3'), which codes for the MBP linker region containing an XmnI and NcoI site fused in frame to the MT gene, and the reverse primer (5'-GACT CTAGAGGATCCTAGGCACAGCACGTG-3' ), which contains the MT gene stop codon and a subsequent BamHI site. Both the PCR product and pMAL-c2x vector were digested with XmnI and BamHI and were later ligated together to generate the final plasmid. In addition to this plasmid Addgene offers versions with 2 copies (32102), 3 copies (32115) and 4 copies (32100) of MT
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
