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URL: http://www.addgene.org/31845
Proper Citation: RRID:Addgene_31845
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20498046
Vector Backbone Description: Backbone Marker:Addgene plasmid #21907; Backbone Size:8141; Vector Backbone:pSicoR-mCH; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: The pSicoR-Ef1α-mCh-Puro lentiviral construct was created by replacing the CMV promoter of pSicoR-mCherry (Addgene vector 21907) with Ef1α and adding an in-frame T2A-puromycin-resistance gene cassette following mCherry. A single RNA molecule is generated for mCherry-T2A-puromycin and two polypeptides are produced by the T2A ribosomal skip motif. The shRNA coding oligos are cloned into the HpaI and XhoI restriction sites as described for pSicoR.
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Source: Addgene
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