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URL: http://www.addgene.org/30541
Proper Citation: RRID:Addgene_30541
Insert Name: oChIEF-tdtomato
Organism: Chlamydomonas reinhardtii
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21878344
Vector Backbone Description: Backbone Marker:Addgene plasmid 18917; Backbone Size:5079; Vector Backbone:AAV-FLEX-rev-ChR2-tdtomato; Vector Types:AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Please note this plasmid has a I170V in channelopsin 1. The changed amino acid improves the protein’s function. To minimize recombination, propagate this plasmids in Stbl2 cells from Invitrogen. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. For more information on growth conditions, please see www.addgene.org/18917 as this is the backbone vector. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed. The expected patterns can be calculated from the attached sequence. Please see Reviews in the right column for an image of Addgene's digest with these enzymes.
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Source: Addgene
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