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URL: http://www.addgene.org/29645
Proper Citation: RRID:Addgene_29645
Insert Name: BirA
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2266; Vector Backbone:pENTR1A; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: The BirA gene was amplified from the E.coli TOP10F' strain, sequence confirmed, and functionally tested in E.coli using a protein fused to an AviTag. Please note that using this plasmid in a LR reaction with a Destination vector encoding an N-terminal tag is not recommended as it would not be in frame with the BirA sequence. This plasmid was designed to be used to biotinylate substrates in vivo. To add a tag to BirA, clone the desired tag into the vector itself, rather than using Gateway cloning--this approach will also prevent unnecessary sequence in the expressed protein.
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Source: Addgene
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