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URL: http://www.addgene.org/29420
Proper Citation: RRID:Addgene_29420
Insert Name: Amber-Cerulean-Amber-Venus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:19946626
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3940; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression, Stoichiometry standard; Bacterial Resistance:Kanamycin
Comments: The 1st Amber can be excised using a NheI-BSP E1 double digest; Cerulean by BSP E1-BglII; the 2nd Amber by BglII-SalI and Venus by SalI-BamHI. The full insert can be released by a NheI-BamHI double-digest. Due to the multiple fluorescent protein repeats in this plasmid, Addgene is unable to confirm the entire insert by sequencing. Please see Addgene's diagnostic digest for this plasmid by clicking the Notes from Addgene link above.
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Source: Addgene
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