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URL: http://www.addgene.org/26751
Proper Citation: RRID:Addgene_26751
Insert Name: H2B-Venus CDS
Organism: Homo sapiens
Bacterial Resistance: Nourseothricin (clonNat)
Defining Citation: PMID:20953177
Vector Backbone Description: Backbone Marker:B. Rosen and W.C.Skarnes, unpublished; Backbone Size:4500; Vector Backbone:pL1L2_gt1; Vector Types:Mammalian Expression, Cre/Lox, FLP/FRT; DRE/Rox; Bacterial Resistance:Nourseothricin (clonNat)
Comments: The pDREV plasmid series encode a 5' FRT site and a 3' loxP site for re-engineering of the IKMC knock-out first alleles by dual RMCE (dRMCE) as described in Osterwalder et al. "Dual RMCE for efficient re-engineering of mouse mutant alleles" Nature Methods (2010) DOI 10.1038/nmeth.1521 Protocol for using these plasmids can be found here: http://www.nature.com/protocolexchange/protocols/1906
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Source: Addgene
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