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URL: http://www.addgene.org/253820
Proper Citation: RRID:Addgene_253820
Insert Name: Pmyo-3 daf-16::GFP
Organism: Caenorhabditis elegans
Bacterial Resistance: Ampicillin
Defining Citation: PMID:41773048
Vector Backbone Description: Backbone Size:7400; Vector Backbone:MosSci vector (Punc-119-mcs-att ); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint. There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function. Addgene found the following discrepancies in our result versus the depositor's reference: 2 SNPs in c. Briggsae unc-119(+); one results in premature stop codon Q157STOP 5 SNPs and 3bp indel in daf-16a Comment from depositor: Functionally, the unc-119 Q7R gene does not affect it to rescue animals with unc-119 mutation. So it should be of no concern. The daf-16a SNPs and indel also do not appear to affect its function, expression or its ability to translocate from cytoplasm to nucleus.
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Source: Addgene
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