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URL: http://www.addgene.org/253104
Proper Citation: RRID:Addgene_253104
Insert Name: SpCas9-P2A-RUBY
Organism: Triticum aestivum and Triticum turgidum
Bacterial Resistance: Kanamycin
Vector Backbone Description: Vector Backbone:JD633; Vector Types:Plant Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Please visit https://doi.org/10.64898/2026.02.10.705172 for bioRxiv preprint. The reference articles to construct this plasmid: A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875 A reporter for noninvasively monitoring gene expression and plant transformation. He Y, Zhang T, Sun H, Zhan H, Zhao Y. Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. 10.1038/s41438-020-00390-1 PubMed 33024566
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Source: Addgene
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