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URL: http://www.addgene.org/24967
Proper Citation: RRID:Addgene_24967
Insert Name: insulin-like growth factor 1
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11709186
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: mRNA injection sense: cut with NotI, transcribe with Sp6 Anti-sense in situ probe: cut with EcoRI, transcribe with T7 PCR fragment of IGF1 (EcoRI-XhoI) from stage 27 cDNA inserted into pCS2 (EcoRI-XhoI). Sense: 5'AAA GAA TTC ATG GAA ACA AAC AAC AAT Antisense: 5'-TTT CTC GAG TTA CAT TCG GAA TCC TCG
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
