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URL: http://www.addgene.org/242954
Proper Citation: RRID:Addgene_242954
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4244; Vector Backbone:pAG AB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid 242954 pAG mKappa Empty encodes the mouse kappa light chain constant region and a filler sequence in lieu of a light chain variable region. To clone in your desired light chain variable region in frame with the mouse kappa light chain constant region, Addgene recommends the NEB Hifi assembly method. PCR Amplify the backbone from plasmid 242954 pAG mKappa Empty with the following primers: 5’-cgggctgatgctgcaccaac and 5’-ggccctcgaggacgccagca. Synthesize a gene fragment of the desired light chain variable region with flanking sequences that overlap with the backbone PCR product 5’-tgctggcgtcctcgagggcc-Gene fragment-cgggctgatgctgcaccaac Assemble with the NEB HiFI assembly (NEB #E2621S) following the manufacturer’s recommendations
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
