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URL: http://www.addgene.org/242954
Proper Citation: RRID:Addgene_242954
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:4244; Vector Backbone:pAG AB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid 242954 pAG mKappa Empty encodes the mouse kappa light chain constant region and a filler sequence in lieu of a light chain variable region. To clone in your desired light chain variable region in frame with the mouse kappa light chain constant region, Addgene recommends the NEB Hifi assembly method. PCR Amplify the backbone from plasmid 242954 pAG mKappa Empty with the following primers: 5’-cgggctgatgctgcaccaac and 5’-ggccctcgaggacgccagca. Synthesize a gene fragment of the desired light chain variable region with flanking sequences that overlap with the backbone PCR product 5’-tgctggcgtcctcgagggcc-Gene fragment-cgggctgatgctgcaccaac Assemble with the NEB HiFI assembly (NEB #E2621S) following the manufacturer’s recommendations
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Source: Addgene
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