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URL: http://www.addgene.org/221139
Proper Citation: RRID:Addgene_221139
Insert Name: 3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
Organism: Synthetic
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: RSF1010-based plasmid. Expresses 3xF-BE4-PpAPOBEC1(H122A) from lacIq-Ptac promoter. IPTG induction is required for base editing in Coxiella burnetii. Unique XhoI, NotI and SapI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid encodes sacB, but sacB is not required for Coxiella burnetii base editing protocol. Plasmid alias: pSAST372.
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Source: Addgene
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