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URL: http://www.addgene.org/221138
Proper Citation: RRID:Addgene_221138
Insert Name: 3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
Organism: Synthetic
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:39560384
Vector Backbone Description: Backbone Marker:Matxalen Llosa lab; Vector Backbone:pMTX808; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: RSF1010-based plasmid. Expresses 3xF-HF-BE3 from lacIq-Ptac promoter. Basal expression is sufficient for base editing in Coxiella burnetii. Note: During growth of E. coli harboring this plasmid, in some cells/colonies, the basal expression of 3xF-HF-BE3 can lead to spurious deamination of cytosines on the plasmid. Unique XhoI, NotI and XbaI sites for addition of sgRNA-encoding region(s) from pHelper-CBE-sgRNA (Addgene plasmid #221137) helper plasmid derivatives. Plasmid harbors oriT, but relaxase gene mobA is interrupted by AmpR. Plasmid alias: pSAST356.
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Source: Addgene
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