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URL: http://www.addgene.org/221136
Proper Citation: RRID:Addgene_221136
Insert Name: 3xF-dCas9
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:39560384
Vector Backbone Description: Backbone Marker:Robert Heinzen lab; Vector Backbone:pJB-KAN-3xFLAG; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: RSF1010-based plasmid. Expresses 3xF-dCas9 from cbu1169 promoter. Unique XhoI, NotI and EcoRI sites for addition of sgRNA-encoding region(s) from pHelper-CRISPRi-sgRNA (Addgene plasmid #221135) helper plasmid derivatives. Plasmid alias: pSAST200.
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Source: Addgene
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