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URL: http://www.addgene.org/221128
Proper Citation: RRID:Addgene_221128
Insert Name: FAPoptim
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:38809589
Vector Backbone Description: Vector Backbone:pFA6-KANMX4; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This FAPoptim integration plasmid was constructed by subcloning the FAPoptim sequence into the pFA6a-KANMX4 plasmid described in (Goldstein and McCusker, 1999). The resulting plasmid can be used for C-terminal tagging of endogenous protiens using the PCR-based integration strategy described in (Longtine et al., 1998).
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
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