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URL: http://www.addgene.org/21826
Proper Citation: RRID:Addgene_21826
Insert Name: SV40 Large T
Organism: simian virus
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:3200; Vector Backbone:Bluescribe; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: SV40 large T antigen for immortalizing cells. See Author's Map for more information. Please note that this plasmid likely expreses a truncated SV40 large T antigen protein starting with the methionine at amino acid 109, when compared to GenBank reference sequence YP_003708382.1 (see NC_001669.1 for the corresponding nucleotide sequence). The plasmid functions as described to immortalize MEFs.
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Source: Addgene