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URL: http://www.addgene.org/212122
Proper Citation: RRID:Addgene_212122
Insert Name: SUMO-sfGFP-WT
Bacterial Resistance: Tetracycline
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sumo-sfGFP, uninterrupted by TAG codons, and b) Methanomethylophilus alvus pyrrolysine tRNA(6). It is to be paired with a plasmid that expresses M. alvus pyrrolysyl-tRNA synthetase (MaRS). This pALS3 control plasmid is used during fluorescence-based selection and characterization of Ma RS mutants. The plasmid here is distinguished from a previous Ma version by the presence of a tetracycline efflux marker.
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Source: Addgene
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