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URL: http://www.addgene.org/212121
Proper Citation: RRID:Addgene_212121
Insert Name: sfGFP-150TAG
Bacterial Resistance: Tetracycline
Vector Backbone Description: Backbone Size:4807; Vector Backbone:pALS3; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Comments: This plasmid encodes expression of a) sfGFP with an amber (TAG) codon at N150 and b) Methanomethylophilus alvus tRNA(6). It is to be paired with a plasmid that expresses an M. alvus pyrrolysyl-tRNA synthetase (MaRS). The pALS3 plasmid is used during fluorescence-based selection and characterization of MaRS mutants. It is distinguished from a previous Ma version by containing a tetracycline efflux marker.
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Source: Addgene
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