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URL: http://www.addgene.org/199237
Proper Citation: RRID:Addgene_199237
Insert Name: S. aureus gRNA spacer
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:36928106
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: This gRNA expression construct can be combined with target site-containing donor plasmids AD59_pEP.DonorCLYBL.TS (Addgene plasmid #199227) or AZ64_pE.DonorCLYBL.TS (Addgene plasmid #199228) to insert foreign DNA at the human CLYBL safe harbor locus using SaCas9 nucleases (HMEJ-based gene targeting) or SaCas9.D10A nickases (ITPN-based gene targeting), such as that encoded by Addgene plasmid #199251.
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Source: Addgene
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