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URL: http://www.addgene.org/187823
Proper Citation: RRID:Addgene_187823
Insert Name: TVA-mCherry
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35866706
Vector Backbone Description: Backbone Size:3706; Vector Backbone:5xUAS Tol2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://doi.org/10.1101/2021.03.25.436574 for bioRxiv preprint. DNA construction: TVA-mCherry was amplified by PCR from a plasmid (gift from Dr. Uchida, Addgene plasmid #38044 ; http://n2t.net/addgene:38044 ; RRID:Addgene_38044) and inserted into a 5xUAS vector (Asakawa, K. et al. Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish. Proc. Natl. Acad. Sci. U. S. A. 105, 1255–1260 (2008).). Used to create transgenic Zebrafish and make use of the Gal4/UAS system. Note: Addgene's NGS results identified several discrepancies with the depositor's in silico generated reference sequences. The depositing lab has confirmed the discrepancies and confirmed that the plasmid functions described in the associated publication.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
