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URL: http://www.addgene.org/182363
Proper Citation: RRID:Addgene_182363
Insert Name: 4x miR-124 target sites
Organism: Synthetic
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Four copies of miR-124 target sites (Åkerblom et al., 2012, https://doi.org/10.1523/JNEUROSCI.0558-12.2012) were cloned into “StLa” reporter plasmid (pCAG-loxP-pEM7-Neo*-bGHpA-3xSV40pA-loxP-tauLacZ-bGHpA from Nam and Benezra, 2009, https://doi.org/10.1016/j.stem.2009.08.017) without the tauLacZ insert and the Rosa26 homology arms. The 4x miR-124 target sequence can be released with BamHI and NotI. More information on the “StLa” reporter at (1) http://www.informatics.jax.org/allele/MGI:4366911 (2) https://figshare.com/ndownloader/files/38884434 (clicking this link will download a file)
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Source: Addgene
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