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Plasmid Name
pMC2m
RRID:Addgene_17970 RRID Copied  
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RRID:Addgene_17970
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Plasmid Information

URL: http://www.addgene.org/17970

Proper Citation: RRID:Addgene_17970

Insert Name: tetR (B)

Bacterial Resistance: Kanamycin

Defining Citation: PMID:17483222

Vector Backbone Description: Backbone Size:0; Vector Backbone:pMV306; Vector Types:mycobacteria expression; Bacterial Resistance:Kanamycin

Comments: The N-terminus (first 50 AA) of TetR are codon usage adapted for mycobacteria. tetR is expressed from a mycobacterial promoter of intermediate strength (pimyc = ptb21). Also see Pubmed ID's 18059281 and 15687379.

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Data and Source Information

Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org