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URL: http://www.addgene.org/17705
Proper Citation: RRID:Addgene_17705
Insert Name: CBFRE
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17721509
Vector Backbone Description: Backbone Size:3600; Vector Backbone:na; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Comments: CBFRE: four CBF1-binding elements from EBV (see Hsieh MCB 1996, 16:952) and the basal simian virus 40 (SV40) promoter upstream of EGFP. The sequence of the CBF1 binding element is GATCTGGAGTAACACGCCGTGGGAAAAAATTTATG. The binding site within this sequence is GTGGGAA. A SmaI+XbaI double digest should yield 3.6kb and approx. 1.2kb bands. See Reviews link in right-hand column to view Addgene's diagnostic digest of this plasmid.
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Source: Addgene