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URL: http://www.addgene.org/17703
Proper Citation: RRID:Addgene_17703
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10839358
Vector Backbone Description: Backbone Size:7600; Vector Backbone:pCLE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: pCLE includes a modified 5' LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).
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Source: Addgene
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