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URL: http://www.addgene.org/17693
Proper Citation: RRID:Addgene_17693
Insert Name: PTPalpha Y789F
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10698938
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5178; Vector Backbone:pTet-splice; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: PTPalpha(Y789F)-HA was generated using pXJ41PTPalpha as a template with the 5' primer 5'-CGCCAAGCTTGGCCACCATGGATTCCTGGTTCATTCTTGT-3' and the 3' primer 5'-TGTCAAGCTTCATGCGTAGTCTGGCACGTCGTATGGGTACTTGAAGTTGGCAAAATCTGA-3'. The substitution ATA->AAA results in a Tyr789->Phe substitution. HindIII-cleaved PCR products were ligated into pTet-Splice. The plasmid contains a CMV promoter under control of regulatory sequences from the tet operon such that, when cotransfected with the transactivator pTet-tTAk, it expresses protein only in the absence of doxycycline.
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Source: Addgene
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