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URL: http://www.addgene.org/176893
Proper Citation: RRID:Addgene_176893
Insert Name: moxGFP-P2A-T2A
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34877484
Vector Backbone Description: Backbone Marker:Eric Kowarz; Backbone Size:5857; Vector Backbone:pSBbi-Pur (Addgene Plasmid #60523); Vector Types:Mammalian Expression, Transposon; Bacterial Resistance:Ampicillin
Comments: The moxGFP-P2A-T2A construct was designed by Jorge L. Arias-Arias from Universidad de Costa Rica ([email protected]). It was codon optimized for human and mouse expression and includes two additional cloning sites (5' EcoRV - 3' SalI and 5' SmaI - 3' XbaI, see the map uploaded by the depositor) for the polycistronic expression of up to three genes: moxGFP and two more. This vector is suitable for transient transfection, but it was intended to be co-transfected with the transposase vector pCMV(CAT)T7-SB100 (Addgene Plasmid #34879) in order to generate mammalian cells with stable expression of the genes of interest. Enjoy!
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Source: Addgene