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Plasmid Name
EF.hICN1.CMV.GFP
RRID:Addgene_17623 RRID Copied  
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RRID:Addgene_17623
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Plasmid Information

URL: http://www.addgene.org/17623

Proper Citation: RRID:Addgene_17623

Insert Name: ICN1

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:16513761

Vector Backbone Description: Backbone Marker:Linzhao Cheng Lab; Backbone Size:8200; Vector Backbone:EF.v-CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin

Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function. The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter. Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.

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Data and Source Information

Source: Addgene