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URL: http://www.addgene.org/17074
Proper Citation: RRID:Addgene_17074
Insert Name: Prickle-1
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12699626
Vector Backbone Description: Backbone Size:0; Vector Backbone:CS2+GFP-BB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR. Species of Sequence Origin: zebrafish. Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp. Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).
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No rating or validation information has been found for ZE33 Prickle-1-GFPnopren-pCS2+.
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Source: Addgene
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