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URL: http://www.addgene.org/17043
Proper Citation: RRID:Addgene_17043
Insert Name: wnt-4 cDNA
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8541205
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Insert: wnt-4 (zebrafish) cDNA. Insert size: 1.3 Kb. To make an in situ probe, linearize with EcoRV and transcribe with SP6. To make sense RNA, linearize with Xba1 and transcribe with T7. Plasmid History: Constructed by Anne Ungar on date of 6/8/93. Species of Sequence Origin: Zebrafish. Notes for Use: Note presence of R1, Sma, BamH1 sites brought in from pBSSK during construction. The 1274 nucleotide Nsi1 fragment containing the complete wnt-4 coding region was ligated into the Pst1 site of pBSSK (zf4 in pBSSK clone 5). The 5' end is near the Kpn1 site. A 1.3 Kb fragment was cut out with EcoRV and Spe1, isolated and ligated into EcoRV/Spe1 cut pT7Ts. Citation: Ungar et al., Mechanisms of Development 52 (1995)
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Source: Addgene
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