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URL: http://www.addgene.org/170273
Proper Citation: RRID:Addgene_170273
Insert Name: NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
Organism: Bradyrhizobium sp. (strain ORS 278)
Bacterial Resistance: Kanamycin
Defining Citation: PMID:34162879
Vector Backbone Description: Backbone Marker:Originally derived from pEGFP-N1 backbone after several modifications in our lab.; Backbone Size:3427; Vector Backbone:pQP-Select; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: To design iLight, a full-length bacterial phytochrome IsPadC from Idiomarina sp. A28L was used as an original template. First, diguanylyl cyclase effector in IsPadC was removed, then following mutations were introduced: I68F, H80Q, A86T, R90S S242C, E274K, R295H I360V, and L464V. After that, iLight was human codon optimized for mammalian expression.
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Source: Addgene
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