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URL: http://www.addgene.org/170270
Proper Citation: RRID:Addgene_170270
Insert Name: iLight
Organism: Idiomarina sp. A28L
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34162879
Vector Backbone Description: Backbone Marker:Life Technologies-Invitrogen (partially modified); Backbone Size:3999; Vector Backbone:Modified pBAD/His-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To design iLight for expression in bacteria, a full-length bacterial phytochrome IsPadC from Idiomarina sp. A28L was used as an original template. First, diguanylyl cyclase effector in IsPadC was removed, then following mutations were introduced: I68F, H80Q, A86T, R90S S242C, E274K, R295H I360V, and L464V.
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Source: Addgene
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