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URL: http://www.addgene.org/170103
Proper Citation: RRID:Addgene_170103
Insert Name: bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:33927418
Vector Backbone Description: Backbone Size:5238; Vector Backbone:pET; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: This is a bacterial codon-optimized SpCas9 (H840A) sequence within pET-28b backbone (Addgene #73018) fused to bipartite NLS, a 33-amino acid linker and the engineered M-MLV RT from the parental pCMV-PE2 plasmid (Addgene #132775). To this construct, a short GS linker and a 6xHis-tag are added to C terminus.
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Source: Addgene
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