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URL: http://www.addgene.org/16995
Proper Citation: RRID:Addgene_16995
Insert Name: Xbhh-C
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:7671801
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Expression ConstructXenopus Banded Hedgehog. (a.k.a. Xbhh; hh1) Insert: PCR of coding region, Genbank accession number U26404. Antisense RNA: For in situ we use clone XIL in Bluescript KS. Linearize with Bam H1 and transcribe with T3. Sense RNA: Linearize with Bam H1 and transcribe with T7. References: Ekker et al., Development 121, 2337-2347 (1995) Lai et al., Development 121, 2349-2360 (1995) Subclone Xbhh-N in pT7Ts. Encodes amino terminal product with autoproteolytic cleavage. Linearize with BamH1 and transcribe with T7. Subclone Xbhh-dominant negative-C in pT7TS: Encodes a leader sequence, a few amino acids amino terminal to the autoproteolytic site, and the carboxy-terminal domain. Linearize with BamH1 and transcribe with T7.
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Source: Addgene
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