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URL: http://www.addgene.org/16985
Proper Citation: RRID:Addgene_16985
Insert Name: Xwnt-5A
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8275867
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pSP64T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: cDNA from Melton lambda gt10 oocyte library. Transcription: linearize with Xba1 and transcribe with SP6. Plasmid History: Constructed by Randall Moon on date of 9/15/90. Notes for Use: EcoR1 insert of lambda G6 of 2/11/90. Contains significant 5' UTR and is less translatable in vitro per ng RNA than Xwnt-5A Hind III/SP64T. However, the type of activity is the same in both constructs. Citation: Moon et al., Development 119, 97 (1993)
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Source: Addgene