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URL: http://www.addgene.org/16897
Proper Citation: RRID:Addgene_16897
Insert Name: Prickle-1
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12699626
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T easy; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Full length prickle-1 with 42bp of 5' UTR, cloned into pGEM-Teasy. The construct makes good in situ probes but does not translate in vitro due to an atg in the polylinker. To make antisense probe: cut with Nco1, transcribe with SP6. For the sense strand cut with Nde1 and transcribe with T7. Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).
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Source: Addgene
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