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Plasmid Name
pBluescript Sp5
RRID:Addgene_16884 RRID Copied  
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RRID:Addgene_16884
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Plasmid Information

URL: http://www.addgene.org/16884

Proper Citation: RRID:Addgene_16884

Insert Name: sp5

Organism: Danio rerio

Bacterial Resistance: Ampicillin

Defining Citation: PMID:11641225

Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: Zebrafish sp5 (bts1) cDNA Full-length in pBluescript SK, cloned into EcoRI / XhoI. Useful for making antisense probe. Is the paralog of sp5-like (sp5l, clone 27-i2 from the Wnt8 upregulated microarray screen). The clone comes from library screening (library from Bruce Appel) and the library was constructed using EcoRI and XhoI linkers; The library was made into the Uni-ZAP XR Lambda vector, and from it Bluescript SK was excised.

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Data and Source Information

Source: Addgene