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Plasmid Name
pGem-LP1-loxP-bar-lox2272-71
RRID:Addgene_168785 RRID Copied  
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RRID:Addgene_168785
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Plasmid Information

URL: http://www.addgene.org/168785

Proper Citation: RRID:Addgene_168785

Insert Name: bar

Organism: Synthetic

Bacterial Resistance: Ampicillin

Defining Citation: PMID:35168324

Vector Backbone Description: Backbone Size:3033; Vector Backbone:pGEM; Vector Types:Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin

Comments: Vector used to create Aspergillus nidulans parental strains with LP1 by polyethylene glycol (PEG)-calcium-based transformation with NotI linearized vector pGemLP1-loxP-bar-Lox2272-71 containing 1 kb homology regions at 5′ and 3′ of the floxed bar to facilitate homologous recombination in A. nidulans LO8030 ∆St locus. Colonies are selected for resistance to glufosinate extracted from Basta.

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Data and Source Information

Source: Addgene