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URL: http://www.addgene.org/168784
Proper Citation: RRID:Addgene_168784
Insert Name: Cre
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35168324
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2812; Vector Backbone:pGem; Vector Types:Cre/Lox, Synthetic Biology, Expression in filamentous fungi; Bacterial Resistance:Ampicillin
Comments: For transient Cre recombinase expression in Aspergillus nidulans, we created the helper vector pGem-PgpdA-Cre-TtrpC unable to replicate in filamentous fungi. The helper vector encodes a cassette for constitutive expression of Cre under the promoter gpdA (PgpdA) and the terminator trpC . As the recipient strain A. nidulans LP1 carries nkuA∆, which minimises random integration events, the helper vector presumably would be lost during fungal growth
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Source: Addgene
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