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URL: http://www.addgene.org/16855
Proper Citation: RRID:Addgene_16855
Insert Name: Wnt-1-myc
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:2673541
Vector Backbone Description: Backbone Size:2746; Vector Backbone:pGEM4; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Description: Full coding sequence of mouse Wnt-1 (int-1 in early literature), with one internal c-myc epitope. Protein can be immunoprecipitated, recognized in whole mount immunostaining, and detected by western blot, using the anti-c-myc antibody 9E-10. Species of Sequence Origin: mouse. Notes for Use: Linearize with Eco R1 and transcribe with T7 to make RNA. Reference: This is the clone published as p284 in AP McMahon and RT Moon, Cell 58: 1075-1084 (1989).
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Source: Addgene