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URL: http://www.addgene.org/167824
Proper Citation: RRID:Addgene_167824
Insert Name: EKAREN4
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33795873
Vector Backbone Description: Backbone Size:3450; Vector Backbone:miniTol2 expression vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: We received EKAREV FRET biosensor from Matsuda-lab (Japan). We subsequently improved by insertion of a Turquoise2 synthetic DNA, including 169 silent mutations (reducing recombination) and adapted the sensor domain by point mutagenesis. The generation of stable cell lines using this plasmid requires the co-transfection of a transposase-expressing plasmid. The depositing lab recommends the use of Plasmid 158774.
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No rating or validation information has been found for miniTol2 x EF1a_EKAREN4 + PGK-puro.
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Source: Addgene
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