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URL: http://www.addgene.org/165523
Proper Citation: RRID:Addgene_165523
Insert Name: Tli DNA polymerase (exo-)
Organism: Other
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: DNA polymerase; lacks 3' to 5' exonuclease activity. Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the 'homing site' in gene that lack the intein. Comparable to VentR (exo-) DNA Polymerase at NEB. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
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Source: Addgene
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