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URL: http://www.addgene.org/16076
Proper Citation: RRID:Addgene_16076
Insert Name: HSP33 cys
Organism: E. coli
Bacterial Resistance: Kanamycin
Defining Citation: PMID:16054089
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5190; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The HSP-FRET probe was generated by inserting YFP into the pECFP-N1 plasmid between the NheI and BglII sites and then ligating the redox-sensitive regulatory domain from the Escherichia coli HSP-33 between YFP and CFP via the EcoRI and BamHI sites. HSP-33: 210 amino acids; BA000007 region 4241962-4242826. Made in Hammerling lab; used in Waypa et al., Increases in mitochondrial reactive oxygen species trigger hypoxia-induced calcium responses in pulmonary artery smooth muscle cells, Circ Res. 2006 Oct 27; 99(9):970-8 PMID: 17008601.
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Source: Addgene
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