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URL: http://www.addgene.org/15899
Proper Citation: RRID:Addgene_15899
Insert Name: RhoA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10087266
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: There is an additional F25N mutation in the sequence. This change greatly facilitated expression of the protein in E.coli. The only known effect of this mutation may be negatively affect the transduction of RhoA signaling to DGK1 kinase. Otherwise the depositing lab have never found any discrepancies with other published work on activity or protein interactions.
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Source: Addgene