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URL: http://www.addgene.org/155130
Proper Citation: RRID:Addgene_155130
Insert Name: SARS-CoV-2 Spike-delta21
Organism: Severe acute respiratory syndrome coronavirus 2
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32384820
Vector Backbone Description: Backbone Size:4750; Vector Backbone:HDM; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Based on a plasmid described previously in Crawford et al. 2020, Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays (https://www.mdpi.com/1999-4915/12/5/513). This is a version of the Spike expression plasmid used to pseudotype lentivirus which provided better titers in this protocol.
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Source: Addgene
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