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URL: http://www.addgene.org/15135
Proper Citation: RRID:Addgene_15135
Insert Name: nLacZ
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12413898
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pRAVE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: In addition to the long terminal repeat (LTR) promoter, the RAVE-nlacZ viral vector has an internal Xenopus elongation factor alpha (EF1-alpha) promoter. To construct RAVE-nlacZ, the Xenopus EF1-alpha gene from pEF-puro (from Gord Fishell), was cloned into the pRIA vector.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org