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URL: http://www.addgene.org/14865
Proper Citation: RRID:Addgene_14865
Insert Name: DAG reporter
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12782683
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: CFP and YFP fused to the C1A and C1B domains of PKCbetaII. DAGR was created by PCR of the C1A and C1B domains of rat PKCßII (aa 37–152) from KpnI to XhoI and substitution for the PH domain in CYPHR.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org