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URL: http://www.addgene.org/135188
Proper Citation: RRID:Addgene_135188
Insert Name: CmR
Organism: Synthetic
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:29065183
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Contains two multiple cloning sites for insertion of homologous regions for targeted deletion via lamba red system in E. coli. Contains I-CreI flanking the Cm cassette outside of the flanking multiple cloning sites for excision of homologous regions and CmR marker from the plasmid. Contains I-SceI sites flanking the Cm cassette to allow for excision of the Cm cassette after integration into the E. coli genome for markerless deletion.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
