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URL: http://www.addgene.org/131149
Proper Citation: RRID:Addgene_131149
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Vector Backbone Description: Backbone Marker:Szilvia Kiriakov; Backbone Size:10377; Vector Backbone:pSK247; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: This is the empty dual yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK234. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with an expression clone resulting from LR reaction using pGAN147 basic yTRAP sensor.
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Source: Addgene
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