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URL: http://www.addgene.org/125258
Proper Citation: RRID:Addgene_125258
Insert Name: single chromosomal copy of yhhX target sequence upstream from mcherry reporter gene under control of a constitutive promoter
Bacterial Resistance: None
Defining Citation: PMID:31377338
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:None
Comments: Genotype: The expression of mcherry can be visualized or measured with a spectrophotometer. The yhhX target sequence followed by a mcherry reporter gene carried by an integrative vector based on pOSIP-KL was integrated at the lambda attB site in the chromosome of E. coli MG1655 and the backbone was flipped out using the pE-FLP (AmpR, #45978, Addgene) plasmid. This strain can be used to optimize dCas9 expression levels
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Source: Addgene
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